WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 1 of 10 This is important but long so follow TL / DR if you don't have time. Firstly, I am an organic chemist (and something of a psychonaut) with access to a decent suite of analytics (GCMS, LCMS, NMR etc. ). Secondly, there are some rumours that there are ‘fake’ lyse rgamide blotters circulating. I felt inclined to do an experiment with some of our favourite molecules, the lysergamides! 1P - LSD, 1cP - MIPLA, LSZ and 1cP - AL - LAD blotters used in this test were purchased from a single vendor — the reported fake/counterfeit blotters. This vendor does not have the best track record (although they are one of the larger vendors), and it was curious that they were indeed offering blotters with a different design [see images below] compared to the ones we all know and trust from t he main lysergamide lab [see images below]. This, coupled with a curious newsletter the main lysergamide lab published a few months back (more on that later), got my chemistry senses tingling even more and led me to do a proper investigation WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 2 of 10 Experiment The samples were prepared by taking two blotters of each product and cutting into tiny slithers (around 30 – 5 0 per blotter). These were added to an LCMS vial and covered with methanol (around 1.5 m l) be fore sonication for 1 – 2 hrs. The sonication process ensures efficient blotter extraction. Methanol was chosen since all the compounds are fairly well soluble in it. The methanol extract was then transferred to fresh LCMS vials b y filtering through a 0. 22 μm syringe filter to remove any particles. Each product was processed in the exact same manner, such that I had separate vials of methanol extract containing test samples of 1P - LSD, 1cP - MIPLA, LSZ and 1 cP - AL - LAD. Additionally, I had two methanol ex tracted reference blotter samples of 1P - LSD and 1cP - AL - LAD from blotters purchased from the main lysergamide producer. These samples were then run on a 2019 model Perkin Elmer LCMS instrument (technically it’s LC - UV - MS) running Epower software for data analysis/interpretation. The column installed is a fairly decent Dr Maisch C18 5 μm 150 m m column. Eluent system A is 0.1% formic acid in water and B is 0.1% formic acid in acetonitrile (I chose an acidic WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 3 of 10 system to prevent hydrolysis of the N - acyl groups). The method was a pretty standard binary gradient going from 90% A to 10% A over 23 minutes and then the gradient was held at 10% A for a further 2 minutes. Elution flow rate was 1 m l/min. Wavelengths measured were 245 n m to 380 n m, including a maxplot of wavelengths. If more details are needed here, I can expand. After data acquisition was complete, I interpreted the data. For NH lysergamides (LSZ only in this case), 313 n m gives the most reliable response. For N - acyl lysergamides (the rest), 292 n m gives the most reliable response. I chose these wavelengths due to my prior experience with them and ergolines. Please see the annotated chromatograms below. I’ve integrated peaks the best I can, given the state of some sam ples, and I’ve tried to make it clear which peak corresponds to which product (where that can be elucidated). Breakdown of the results 1cP - AL - LAD 150 μg blotters ( Figure 2 ) : by far the dirtiest sample tested. I could not even integrate all the peaks otherwise the chromatogram would become illegible. At best, the blotters contain approximately 52% 1cP - AL - LAD (probably much less if I got all the baseline peaks as well). The rest is iso - 1cP - AL - LAD and other unknown impurities. LSZ 150 μg blotters ( Figure 4 ) : also pretty dirty. The major product is approximately 72% trans - LSZ (we don’t know about the S/R configuration though), other impurities include inactive or at least significantly less active isomers such as cis - LSZ, iso - cis - LSZ, iso - trans - LSZ. 1cP - MIPLA 200 μg blotters ( Figure 3 ) : fairly dirty as well. Major product is approximately 78% 1cP - MIPLA but it still contains 20% of iso - 1cP - MIPLA and another 1.5% of unidentifiable impurities. 1P - LSD 100 μg blotters ( Figure 1 ) : fairly dirty as well. Major product is approximately 78% 1P - LSD but it still contains 18% of iso - 1P - LSD. 1% LSD and 2.5% of unidentifi able impurities. Most concerningly, these blotters contain 1% LSD which will mean they are technically illegal pretty much everywhere. Now for the reference samples by our good friends (the tried - and - true blotters on the market and the ones most vendors s till offer); 1P - LSD 100 μg reference blotter ( Figure 5 ) : very clean, almost a single peak. Sample composed of >99% 1P - LSD and a negligible trace of iso - 1P - LSD. 1cP - AL - LAD 150 μg reference blotter ( Figure 6 ) : very clean, almost a single peak. Sample composed of >99% 1cP - AL - LAD and a negligible quantity of unidentifiable impurities. WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 4 of 10 Figure 1 : Analysis of fake 1 P - LSD 1 0 0 μg blotters WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 5 of 10 Figure 2 : Analysis of fake 1 cP - A L - LAD 150 μ g blotter s WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 6 of 10 Figure 3 : Analysis of fake 1 c P - MIPLA 2 00 μg blotters WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 7 of 10 Figure 4 : Analysis of fake LSZ 150 μg blotters WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 8 of 10 Figure 5 : Analysis of reference 1 P - LSD 1 0 0 μg blotters WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 9 of 10 Figure 6 : Analysis of reference 1 c P - AL - LAD 1 5 0 μg blotters WARNING: Very bad quality lysergamide blotters on the market (1P - LSD, 1cP - MIPLA, 1cP - AL - LAD, LSZ) — detailed LCMS analysis Posted by u/anony mouse8691 on 2021 - 03 - 07 p age 10 of 10 In summary The bad quality blotters do really deserve the ‘fake’ tag, at the very least due to the fact that they will contain less active compound than advertised due to the impurities. It is clear that the producer has not made any effort to separate the isomers fo rmed during synthesis - many of which have been reported to cause stomach cramps/discomfort and gas. Furthermore, there are large quantities of unidentifiable impurities in many samples which could make (especially regular) consumption hazardous. Lysergami de chemistry is really quite an art/pain in the ass and obtaining pure product takes skill, persistence and motivation. It is clear to me that this producer is just taking shortcuts to make quick profit. If he cared about the users, he would not serve this unpurified crude product — basically a half - baked cockroach pie! I hope my experiment was clear enough for you. I think this pretty much sums it up. Buy trash blotters, receive trash product. I will be sticking with the blotters supplied by the original lysergamide producers. I suggest others do the same! Also, in case you’re wondering if the isomer could be produced by the sample preparation procedure or analytical technique; that is why I used the reference blotters under the exact same conditions. No isomerisation with the reference blotters indicates that the isomers identified in the test samples can’t be a result of the sample preparation process or analytical technique. Now, my final words. Earlier, I mentioned that there was a newsletter publishe d by the main lysergamide lab, and this was one of the reasons that I embarked on this experiment. Unfortunately, I can’t publish everything they said here, but they wrote about an ex - employee that had gone rogue, done the dirty on them and stolen their mo lecules and synthesis procedures. I am making an educated guess that this person is the same one making these poor quality lysergamide blotters and selling them to an unscrupulous vendor. It all fits perfectly; the time scales (since that newsletter), the shady vendor getting on board and the rumours of bunk/weak blotters. TL / DR There are now dodgy lysergamide blotters on the market. Buy legit lysergamide blotters with the classic print on them (see photo above) and better yet, stick to vendors on the reta iler list on the producer’s website. It is not out of the question that we could even see the same low - quality products (and possibly more to follow) on identical blotter designs, i.e. complete imitations of the original products, which is another reason t o only buy from the trusted retailers. Any questions, please fire away!