WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis This is important but long so follow TL/DR if you don't have time. Firstly, I am an organic chemist (and something of a psychonaut) with 1P-LSD, 1cP-MIPLA, LSZ and 1cP-AL-LAD blotters used in this test were access to a decent suite of analytics (GCMS, LCMS, NMR etc.). purchased from a single vendor—the reported fake/counterfeit Secondly, there are some rumours that there are ‘fake’ lysergamide blotters. This vendor does not have the best track record (although blotters circulating. I felt inclined to do an experiment with some of our they are one of the larger vendors), and it was curious that they were favourite molecules, the lysergamides! indeed offering blotters with a different design [see images below] compared to the ones we all know and trust from the main lysergamide lab [see images below]. This, coupled with a curious newsletter the main lysergamide lab published a few months back (more on that later), got my chemistry senses tingling even more and led me to do a proper investigation Posted by u/anonymouse8691 on 2021-03-07 page 1 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Experiment The methanol extract was then transferred to fresh LCMS vials by filtering through a 0.22 µm syringe filter to remove any particles. The samples were prepared by taking two blotters of each product and cutting into tiny slithers (around 30–50 per blotter). These were added to an LCMS vial and covered with methanol (around 1.5 ml) before sonication for 1–2 hrs. The sonication process ensures efficient blotter extraction. Methanol was chosen since all the compounds are fairly well soluble in it. Each product was processed in the exact same manner, such that I had separate vials of methanol extract containing test samples of 1P-LSD, 1cP-MIPLA, LSZ and 1cP-AL-LAD. Additionally, I had two methanol extracted reference blotter samples of 1P-LSD and 1cP-AL-LAD from blotters purchased from the main lysergamide producer. These samples were then run on a 2019 model Perkin Elmer LCMS instrument (technically it’s LC-UV-MS) running Epower software for data analysis/interpretation. The column installed is a fairly decent Dr Maisch C18 5 µm 150 mm column. Eluent system A is 0.1% formic acid in water and B is 0.1% formic acid in acetonitrile (I chose an acidic Posted by u/anonymouse8691 on 2021-03-07 page 2 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis system to prevent hydrolysis of the N-acyl groups). The method was a LSZ 150 µg blotters (Figure 4): also pretty dirty. The major product is pretty standard binary gradient going from 90% A to 10% A over 23 approximately 72% trans-LSZ (we don’t know about the S/R minutes and then the gradient was held at 10% A for a further 2 configuration though), other impurities include inactive or at least minutes. Elution flow rate was 1 ml/min. Wavelengths measured were significantly less active isomers such as cis-LSZ, iso-cis-LSZ, iso-trans- 245 nm to 380 nm, including a maxplot of wavelengths. If more details LSZ. are needed here, I can expand. 1cP-MIPLA 200 µg blotters (Figure 3): fairly dirty as well. Major product After data acquisition was complete, I interpreted the data. For NH is approximately 78% 1cP-MIPLA but it still contains 20% of iso-1cP- lysergamides (LSZ only in this case), 313 nm gives the most reliable MIPLA and another 1.5% of unidentifiable impurities. response. For N-acyl lysergamides (the rest), 292 nm gives the most 1P-LSD 100 µg blotters (Figure 1): fairly dirty as well. Major product is reliable response. I chose these wavelengths due to my prior approximately 78% 1P-LSD but it still contains 18% of iso-1P-LSD. 1% experience with them and ergolines. LSD and 2.5% of unidentifiable impurities. Most concerningly, these Please see the annotated chromatograms below. I’ve integrated peaks blotters contain 1% LSD which will mean they are technically illegal the best I can, given the state of some samples, and I’ve tried to make pretty much everywhere. it clear which peak corresponds to which product (where that can be Now for the reference samples by our good friends (the tried-and-true elucidated). blotters on the market and the ones most vendors still offer); Breakdown of the results 1P-LSD 100 µg reference blotter (Figure 5): very clean, almost a single peak. Sample composed of >99% 1P-LSD and a negligible trace of iso- 1cP-AL-LAD 150 µg blotters (Figure 2): by far the dirtiest sample tested. 1P-LSD. I could not even integrate all the peaks otherwise the chromatogram 1cP-AL-LAD 150 µg reference blotter (Figure 6): very clean, almost a would become illegible. At best, the blotters contain approximately single peak. Sample composed of >99% 1cP-AL-LAD and a negligible 52% 1cP-AL-LAD (probably much less if I got all the baseline peaks as quantity of unidentifiable impurities. well). The rest is iso-1cP-AL-LAD and other unknown impurities. Posted by u/anonymouse8691 on 2021-03-07 page 3 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 1: Analysis of fake 1P-LSD 100 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 4 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 2: Analysis of fake 1cP-AL-LAD 150 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 5 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 3: Analysis of fake 1cP-MIPLA 200 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 6 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 4: Analysis of fake LSZ 150 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 7 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 5: Analysis of reference 1P-LSD 100 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 8 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis Figure 6: Analysis of reference 1cP-AL-LAD 150 µg blotters Posted by u/anonymouse8691 on 2021-03-07 page 9 of 10 WARNING: Very bad quality lysergamide blotters on the market (1P-LSD, 1cP-MIPLA, 1cP-AL-LAD, LSZ)—detailed LCMS analysis In summary everything they said here, but they wrote about an ex-employee that had gone rogue, done the dirty on them and stolen their molecules and The bad quality blotters do really deserve the ‘fake’ tag, at the very synthesis procedures. I am making an educated guess that this person least due to the fact that they will contain less active compound than is the same one making these poor quality lysergamide blotters and advertised due to the impurities. It is clear that the producer has not selling them to an unscrupulous vendor. It all fits perfectly; the time made any effort to separate the isomers formed during synthesis - scales (since that newsletter), the shady vendor getting on board and many of which have been reported to cause stomach the rumours of bunk/weak blotters. cramps/discomfort and gas. Furthermore, there are large quantities of unidentifiable impurities in many samples which could make TL/DR (especially regular) consumption hazardous. Lysergamide chemistry is really quite an art/pain in the ass and obtaining pure product takes skill, There are now dodgy lysergamide blotters on the market. Buy legit persistence and motivation. It is clear to me that this producer is just lysergamide blotters with the classic print on them (see photo above) taking shortcuts to make quick profit. If he cared about the users, he and better yet, stick to vendors on the retailer list on the producer’s would not serve this unpurified crude product—basically a half-baked website. It is not out of the question that we could even see the same cockroach pie! low-quality products (and possibly more to follow) on identical blotter designs, i.e. complete imitations of the original products, which is I hope my experiment was clear enough for you. I think this pretty another reason to only buy from the trusted retailers. much sums it up. Buy trash blotters, receive trash product. I will be sticking with the blotters supplied by the original lysergamide Any questions, please fire away! producers. I suggest others do the same! Also, in case you’re wondering if the isomer could be produced by the sample preparation procedure or analytical technique; that is why I used the reference blotters under the exact same conditions. No isomerisation with the reference blotters indicates that the isomers identified in the test samples can’t be a result of the sample preparation process or analytical technique. Now, my final words. Earlier, I mentioned that there was a newsletter published by the main lysergamide lab, and this was one of the reasons that I embarked on this experiment. Unfortunately, I can’t publish Posted by u/anonymouse8691 on 2021-03-07 page 10 of 10
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