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The possibility to integrate biorecognition elements into electrochemical detection systems has opened the way to a new class of powerful analytical devices named electrochemical (EC) biosensors. The first EC biosensors employed enzymes as recognition elements; however this limited their application to redox enzymes and natural or artificial redox substrates or inhibitors. Broadening this to include non-electroactive analytes was later possible thanks to the development of affinity sensors in which specific interactions between biomolecules are exploited for developing highly selective and sensitive biosensors. Presently, the combination of the exceptional molecular recognition capabilities of antibodies and aptamers with the sensitivity, low cost, practicality of use and handiness of electrochemical devices is leading to an impressive development of EC immunosensors and aptasensors that are potentially suitable to detect a wide range of analytes, following a path that is moving alongside the most recent advances in proteomics. Interestingly, with continued improvements and refinements in EC immunosensors based on the use of labels, together with intrinsically electroactive, or those with the ability to interact with electroactive molecules, a new generation of label-free sensors is being developed. This Special Issue takes stock of the state of the art and identifies prospects for EC immuno- and aptasensors, both labeled and label-free. Emphasis is placed on analytical applications for the rapid detection of disease markers and for toxicological and food analyses.

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The rapid increasing of concentrations of anthropologically generated greenhouse gases (primarily CO2) in the atmosphere is responsible for global warming and ocean acidification. The International Panel on Climate Change (IPCC) indicates that carbon capture and storage (CCS) techniques are a necessary measure to reduce greenhouse gas emissions in the short-to-medium term. One of the technological solutions is the long-term storage of CO2 in appropriate geological formations, such as deep saline formations and depleted oil and gas reservoirs. Promising alternative options that guarantee the permanent capture of CO2, although on a smaller scale, are the in-situ and ex-situ fixation of CO2 in the form of inorganic carbonates via the carbonation of mafic and ultramafic rocks and of Mg/Ca-rich fly ash, iron and steel slags, cement waste, and mine tailings. According to this general framework, this Special Issue collects articles covering various aspects of recent scientific advances in the geological and mineralogical sequestration of CO2. In particular, it includes the assessment of the storage potential of candidate injection sites in Croatia, Greece, and Norway; numerical modelling of geochemical–mineralogical reactions and CO2 flow; studies of natural analogues providing information on the processes and the physical–chemical conditions characterizing serpentinite carbonation; and experimental investigations to better understand the effectiveness and mechanisms of geological and mineralogical CO2 sequestration.

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Even today, cardiovascular diseases are the main cause of death worldwide, and therapeutic approaches are very restricted. Due to the limited regenerative capabilities of terminally differentiated cardiomyocytes post injury, new strategies to treat cardiac patients are urgently needed. Post myocardial injury, resident fibroblasts begin to generate the extracellular matrix, resulting in fibrosis, and finally, cardiac failure. Recently, preclinical investigations and clinical trials raised hope in stem cell-based approaches, to be an effective therapy option for these diseases. So far, several types of stem cells have been identified to be promising candidates to be applied for treatment: cardiac progenitor cells, bone marrow derived stem cells, embryonic and induced pluripotent stem cells, as well as their descendants. Furthermore, the innovative techniques of direct cardiac reprogramming of cells offered promising options for cardiovascular research, in vitro and in vivo. Hereby, the investigation of underlying and associated mechanisms triggering the therapeutic effects of stem cell application is of particular importance to improve approaches for heart patients. This Special Issue of Cells provides the latest update in the rapidly developing field of regenerative medicine in cardiology.

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