Microfluidic cell sorter with circularly-arranged pillars Ninad Mehendale1, Oshin Sharma1 and Debjani Paul 1, 2 1 Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, India 2 Wadhwani Centre for Bioengineering, Indian Institute of Technology Bombay, India 1. Pillar - based microfluidic cell sorting Dead-end pillars Deterministic lateral displacement Cross flow OncoBean chip Gifford, Lab chip(2014) Weir type Wilding, Anal Biochem (1998) Inglis, Appl Phys Lett (2009) Chen, Sens Act B (2008) Murlidhar, Small (2014) 2. Our goals 3. Our device geometry • Self unclogging of blocked cells Platelet outlet (X) without reverse flow X • One inlet for whole blood, • Ability to work with whole RBC four outlets for RBC-free X blood X capture platelet collection and one • Device operation without RBC outlet. additional buffer flow Prevent • Multiple rows of pillars • Gravity-driven flow to replace sagging arranged in concentric circles. an external pump X • Appropriate pillar gaps to sort WBC • Small device footprint (< 10 mm RBC Whole blood capture cells by size. X 10 mm) outlet (Z) inlet (Y) 4. Results (a) (b) (c) Clogged RBCs (a) RBC selectivity = • Increase in input flow rate Number of RBCs at RBC outlet decreases RBC separation Number of RBCs at platelet outlet efficiency and selectivity. RBCs 4 (b) RBC seperation efficiency % = can squeeze and pass through the 4 3 Number of RBCs at RBC outlet ∗ 100 pillars at higher pressures. 23 2 Total numbar of RBCs at both outlet • RBC recovery increases with 1 increase in flow rate. 1 (c) RBC recovery % = Cell path tracks (1-4) indicate that RBCs follow Number of RBCs at outlet ∗ 100 the cross-flow after the outermost pillar zone Total number of RBCs at inlet gets clogged (shown by red dotted arrows). Acknowledgement: Kaushalya Hospital, Mumbai and Department of Electronics and Information Technology, Government of India . Contact: debjani.paul@iitb.ac.in
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