CLOG-FREE PASSIVE RADIAL PILLAR DEVICE (RAPID) FOR PLATELET ENRICHMENT { N. M EHENDALE , O. S HARMA , C. D‘C OSTA AND D. PAUL } I NDIAN I NSTITUTE OF T ECHNOLOGY B OMBAY, I NDIA O BJECTIVE Enrichment of platelets from whole blood using a label-free passive microfluidic technique. D EVICE DESIGN COMSOL SIMULATIONS Variation of pressure with radial distance within the first zone of RAPID. The inset shows the surface plot of radial pressure obtained by COMSOL simulation. T IME LAPSE Time lapse images showing the paths followed by platelets(blue track) and RBCs (red and green track). R ESULTS Platelet separation parameters as a function of flow rate in RAPID. The platelet enrichment factor (A) decreases with increase in flow rate. In contrast, the throughput of the device (B) increases with in- crease in flow rate. The recovery (C) remains unaffected by the flow rate. C ONCLUSIONS (A-B) Working principle of RAdial PIllar Device (RAPID). (C) Pro- filometry results. Inset shows microscopic image of RAPID (D) The The device is best suited for lower flow rates (<1 ml/min). This schematic diagram of the experimental set-up. avoids platelet activation and leads to 60X platelet enrichment. A CKOWLEDGEMENTS Centre for Excellence in Nanoelectronics, IIT Bombay and Wadhwani Research Centre for Bioengineering, IIT Bombay
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