Towards a portable radial pillar device (RAPID) for clog-free continuous cell separation Ninad Mehendale, Shilpi Pandey, Oshin Sharma, Misal Choudhary and Debjani Paul Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, INDIA What is RAPID? • Clogging-free operation without buffer flow in a passive pillar-based microfluidic chip • Can separate cells from whole blood without any pre-processing step • Operated the device continuously with whole blood for up to 6 hours RAPID for platelet enrichment RAPID for CTC enrichment Microscope image Operating principle Operating principle Performance metrics Centrifugal prototype Microscope image Motor Coupler Chip holder Base • ~ 60X enrichment of platelets at low flow rates Performance metrics • ~ 95% recovery of platelets at all flow rates • Validated centrifugal prototype with beads • Developing the system to handle higher sample volumes Inlet Platelet outlet after separation Where are we currently? • We have demonstrated clogging-free operation of RAPID for ~ 6 hours using whole blood. • A portable prototype has been developed to eliminate the need for syringe pumps. We thank IIT Bombay for travel support, IMPRINT for project funds, Tata Centre for Technology and Design’s workshop for prototype machining, nanofabrication facility for lithography, and confocal facility (IRCC) for imaging. Contact: debjani.paul@iitb.ac.in
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