a b c d 250K 250K 250K 250K DAPI multiplets 200K 200K 200K 0.19 200K DAPI singlets FSC-H FSC-H SSC-A SSC-A 150K 150K 150K 150K 4.68 NeuN+ Singlets 33.7 100K Nuclei 100K 99.8 100K 100K Debris 64.3 95.1 NeuN- 50K 50K 50K 50K 61.5 0 0 0 0 0 50K 100K 150K 200K 250K 3 3 4 5 3 3 4 5 0 50K 100K 150K 200K 250K -10 0 10 10 10 -10 0 10 10 10 FSC-A FSC-A DAPI NeuN e FSC-A f FSC-A x x SSC-A SSC-A Nuclei Nuclei 250K 250K 92.8 88.0 200K 200K FSC-A FSC-A x x SSC-A SSC-A 150K 150K NeuN+ FSC-H NeuN+ FSC-H 0 94.0 100K 100K Singlets Singlets 100 100 NeuN- NeuN- 50K 50K 100.0 5.01 0 0 3 3 4 5 3 3 4 5 -10 0 10 10 10 DAPI -10 0 10 10 10 DAPI NeuN x NeuN x FSC-H FSC-H DAPI singlets DAPI singlets NeuN- pool 97.3 NeuN+ pool 89.0
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