Use RNase-free reagents, tubes, tips and solution. Use RNase-free pipette tips and tubes for RNA Work. If the reagents and tubes are RNAase-free, these do not need to be autoclaved. All solutions should be made with RNase-free water and used only for RNA work. RNase-free water can be obtained from commercial sources. All solutions should be made with RNase-free water and used only for RNA work. Keep Your Samples Cold Throughout Your Protocol Keep your samples cold throughout your protocol whenever practical, and store purified RNA at –80°C in a dedicated box. If the sample needs to be stabilized and preserve for RNA extraction for longer periods of time, view our RNAIntact Stabilization Solution RNA Extraction All About Successful Protect Yourself with Clean Gloves and a Lab Coat Ensure that you are well protected in the lab by putting on your lab coat and clean gloves to protect samples from RNAses on your skin. Do be careful when touching your face or hair with gloved hands. Remove gloves before touching items that are usually touched with bare hands. Change gloves frequently. Clean Your Work Area and Pipettors Prior to Starting Work Clean your work area and pipettors thoroughly with 70% ethanol and 3% hydrogen peroxide, If not, use our very own RNase Erase decontamination solution to wash your pipettors and work area properly. Practice good sterile technique: when working, keep tubes and bottles closed whenever possible, and avoid coughing, sneezing, or breathing over open containers. www.mpbio.com/sg custserv.ap@mpbio.com +65 6775 0008