© 2022 IJRAR May 2022, Volume 9, Issue 2 www.ijrar.org ( E - ISSN 2348 - 1269, P - ISSN 2349 - 5138 ) IJRAR22B2543 International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org 769 Limnological Study O n Microalgal Flora O f Nakane Lake O f Dhule District O f Maharashtra, India. 1 Dnyanesh Khairnar, 2 Tejas Saner, 3 Nikhil Patil, 4 Pruthviraj Shinde, 5 K aran Patil 12345 Student 1 Department of Microbiology, 1 JET’S Z. B. Patil Collage, Dhule, India Abstract : Algae Play vital role in aquatic ecosystems by forming the energy base of the food web for all aquatic organisms. The present study with relation to Potential of Cyanophyceae, Chlorophyceae, Bacillario p hyceae, Euglenineae, classes of algae carried out for Nakane Lake of Dhule district. In Maharashtra very few workers have paid attention on limnological aspects of algae from lotic & lentic water habitat. These studies of Nakane Lake were carried out during December 2020 to November 2021. Geographically Nakan e Lake is located at latitude 20°53'9" N and longitude 74°43'3" E. Dhule are rich in lentic water Systems Supporting a rich algal flora. Limnological study on micro algal flora of Nakane Lake was carried out by Studying algal & Physico - chemical analysis of water. The Present work enriches our knowledge of algal flora of this District and State Index Terms - Algal flora, Limnology, Dhule, Physico - chemical Parameters, Nakane Lake. I INTRODUCTION Water resources have great importance in human development. It is essential for agriculture, industry and human existence. The algae widely spre a d in aquatic environment and water Play's important role in their growth. In Maharashtra Bodas (1991) made hydrobiological and taxonomical Studies of some lotic and lentic waters in all around Nasik. In India the Pioneer works are th ose of Iyengar (1940) on algal flora of some muddy rainwater Pools. Nandan & Patel (1984) studies on algal flora of wishwamitri river Baroda and Nandan & Patel (1985) Studies on Pollution of Wishwamitri river algal flora in Baroda. Ganpati (1940) on the ec ology of temple tank. Gonzalves and Joshi (1946) and Singh (1960) on the phytoplankton ecology. Shantilal, Hari and Nanabhau (2017) Studied algae from sonv a d Dam of Dhule. Munawar (1970) Studies on freshwater Ponds of Hyderabad. Patil V P (2013) Studies on algal flora of Sulwade barrage of Dhule district. In Maharashtra very few workers have paid attention on limnological aspects. Therefore, the present work was undertaken for limnological Study of microalgal flora from three Possible Point Places of Nakane Lake, Dhule district of Maharashtra. II. MATERIALS AND METHOD The Nakane Lake is situated near Morane & H a ranmal village of Dhule district. topography, latitude 20°53'9" N & longitude 74°43'31" E. Three p ossible sites were selected for purpos e of Collecting the algal and water samples at monthly interval for one year. The Samples for Physicochemical analysis were collected in Pre - washed 1L Plastic bottles. The bottles were rinsed three times with Sample water. The samples were taken from the surface at depth of 1 - 2 feet from three different Points, integrated and a representative Sample was drawn. The sampling was carried out at monthly interval during December 2020 to November 2021. During Sampling we used thermoanemometer to measure air flo w, temperatu re and humidity. The quantitative and qualitative study of 4 groups of algae viz. Cyanophyceae, Chlorophyceae, Bacillario p hyceae, and Euglenineae was made. The algal identification of algal taxa by Comparison with the reference: - Desikacharya (1959); Randhawa (1959); Husked (1930); Patric & Reimer (1996); Prescott (1962); Prescott & vinyard (1965); Philopose (1967); sorode & kamat (1984); Cook (1996). Water Sample were analyzed by standard methods given, by APHA (1975) for determining Physico - chemical analysis. The population density of 4 groups of algae was estimated according to the method given by Whitton (1969) © 2022 IJRAR May 2022, Volume 9, Issue 2 www.ijrar.org ( E - ISSN 2348 - 1269, P - ISSN 2349 - 5138 ) IJRAR22B2543 International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org 770 III. RESULT AND DISCUSSIONS In Present Investigation four groups of algae were Studied from three site of lake viz. NLSs , NLEs, NLNs. The minimum and maximum values of physicochemical parameters of 3 Stations of dam are shown in Table 1. The range and average Population of 4 groups of algal for 3 sites of lake shown in table 2. The Population of 4 groups of algae from 3 sit es of study area is shown in Plate 1. Water temperature Played an important role in Controlling the growth and abundance of algal flora (Nazneen, 1980) & (George, 1961) reported that higher PH Value Promoted the growth of algae as agreed in Present Study, Munawar (1970) reported. that higher concentration of carbonate and total alkalinity favo u red the growth of blue - green algae as agreed with views of Hosmani and Bharati (1980). The Population of euglenoids was less and it was not varied in significance a t all 3 sites. This might be due to lower concentration of organic matter in lake as agreed with Hosmani and Bharati (1975) Free co₂ was recorded at all 3 Sites as it was agreed with earlier views (Gonzalves and Joshi, 1948; Zafar, 1964). In comparison of 4 groups of algae the Population of green and blue - green was greater than other groups of algae in Present study. The Population of Cyanophyceae is maximum up to 36 % and it was dominant in abundance as compared to those of other groups VI. CONCLUSION All the Physical and Chemical Properties of Nakane Lake water were within desirable limits. The results obtained from the Present investigation shall be useful in future management of reservoir. The physicochemical charact eristics of reservoir. water suggested that water is suitable for irrigation purpose and aquatic life. Management of reservoir and biodiversity survey also useful for the researchers for their further research study on the algae and Nakane Lake Table 1: Physico - Chemical parameter at 3sites of Nakane Lake during December 2020 to November 2021. Sr. No Physico Chemical Parameters Stations NLSs NLEs NLNs 1 Water Temperature 20.5 - 29.0°c 21.5 - 30.5°c 23.2 - 28.9°c 2 PH 8.2 - 9.2 8.1 - 8.9 8.1 - 9.4 3 Dissolve Oxygen 2.28 - 5.85 3.22 - 7.21 4.02 - 7.15 4 Free Co2 17.6 - 46.2 14.8 - 19.5 8.5 - 49.8 5 Total Alkalinity 120 - 325 128 - 305 190 - 455 6 Carbonate Absent 40 - 90 20 - 115 7 Bicarbonate 150 - 530 35 - 150 45 - 200 8 Sulphate 1.4 - 2.3 1.4 - 3.5 1.4 - 2.8 9 Phosphate 0.01 - 0.01 0.01 - 0.14 0.01 - 0.14 10 Nitrate 0.06 - 0.11 0.05 - 0.10 0.04 - 0.12 11 Hardness 68 - 105 78 - 112 79 - 120 12 Chloride 35.8 - 114.2 52.01 - 106.3 56.1 - 98.3 13 Magnesium 4.98 - 18.97 2.94 - 14.4 1.05 - 97.4 14 Magnesium Hardness 16.4 - 66.6 8.05 - 53.6 1.96 - 88.3 15 Calcium 10.22 - 28.1 10.45 - 24.3 9.03 - 30.00 16 Calcium Hardness 18.4 - 91.3 20.04 - 91.3 20.02 - 88.6 17 Total Solids 200 - 1230 200 - 1300 100 - 1030 18 Total Dissolved Solid 100 - 910 80 - 590 100 - 890 19 Total Suspended Solid 100 - 950 100 - 800 100 - 990 All parameters are expressed in Mg/ l Except PH and water temperature. NLSs – Nakane Lake South site. NLEs – Nakane Lake East site. NLNs - Nakane Lake North site. Plate 1: Population of four groups of algal from three stations of Nakane Lake. (December 2020 to November 2021) © 2022 IJRAR May 2022, Volume 9, Issue 2 www.ijrar.org ( E - ISSN 2348 - 1269, P - ISSN 2349 - 5138 ) IJRAR22B2543 International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org 771 Table 2: The range and average population of 4 groups of algal from 3 sites of Nakane Lake during December 2020 to November 2021. Algal Groups Stations NLSs NLEs NLNs Cyanophyceae Range 23 - 152 25 - 90 20 - 112 Average 59.32 54.91 56.16 Total population 635 531 503 Chlorophyceae Range 40 - 75.5 32.5 - 60 27.5 - 87.5 Average 55.20 52.91 47.79 Total population 609 466 478 Bacillariophyceae Range 18.5 - 69 15 - 67.5 22.5 - 57 Average 33.02 29.08 38.79 Total population 366 302 412 Euglenineae Range 8.5 - 20 7.5 - 20.5 3.5 - 22.5 Average 14.18 14.54 11.79 Total population 170 163 143 V. ACKNOWLEDGEMENT we would like to express our deep gratitude to Principal P. H. Pawar, Head of Department (microbiology) Dr. R. D. Shelar for Providing laboratory facility during research Period. We also grateful to our lab attendant Mr. G. B. Tadvi REFERENCE [1] American Public Health Association. (1975). Standard methods for the examination of water and wastes including bottom sediments and sludges 14th ed. New York, 769. [2] Bodas, Kanchan (1991). Hydrobiological and taxonomic studies of some lotic and lentic waters in and around Nashik. Bio. L. Ind. 2 (1 & 2 ): 13 - 21 [3] Cook, C. D. (1996). Aquatic and wetland plants of India. Oxford University Press. [4] Desikacharya, T. V. (1959). Cyanophyta ICAR. New Delhi, 686. [5] Ganapati, S. V. (1940). The ecology of a temple tank containing a permanent bloom of Microcystis aeruginosa (Kutz.) Henfr. J. Bombay Nat. Hist. Soc, 42(1), 65 - 77. [6] Gonzalves, E. A., & Joshi, D. B. (1946). Freshwater algae near Bombay. I. The seasonal succession of the algae in a tank at Bandra. J. Bomb. Nat. Hist. Soc, 46(1), 154 - 176. [7] Gunale, V. R., & Balakrishnan, M. S. (1981). Biomonitoring of eutrophication in the Pauna, M ule and Mutha rivers flowing through Poona [India]. Indian journal of environmental health. [8] Hosmani, S. P. (1975). Limnological studies in ponds and lakes of Dharwar. [9] Hosmani, S. P., & Bharati, S. G. (1975). Hydrobiological studies in ponds and lakes of Dharwar, 3 occurrence of twoeuglenoid blooms. J. Karnatak Univ., 20, 151 - 156. [10] Hosmani, S. P., & Bharati, S. G. (1980). Algae as indicators of organic pollution. Phykos, 19(1), 23 - 26. [11] Hustedt, F. (1930). Bacillariophyta (Diatomeae). Susswasser - flora Mitte leuropas, 2. no. 10, 1 - 466. [12] Mahajan, S. R. (2001). Ecological studies of algae from certain lakes of Jalgaon district of Norht Maharashtra (Doctoral dissertation, Ph. D. thesis, North Maharashtra University Jalgaon (MS)). [13] Munawar, M. (1970). Limnological studies on freshwater ponds of Hyderabad - India I. The Biotope. Hydrobiologia, 35(1), 127 - 127. [14] Nandan, S. N., & Patel, R. J. (1984). Ecological studies on algal flora of Vishwamitri River, Baroda, Gujarath. Indian Journa l of Plant Nature, 1, 17 - 32. [15] Nanda n, S. N., & Patel, R. J. (1985). Pollution studies of Vishwamitri river flowing through Baroda. Biovigyanam. [16] Nazneen, S. (1980). Influence of hydrological factors on the seasonal abundance of phytoplankton in Kinjhar Lake, Pakistan. Internationale Revue d er gesamten Hydrobiologie und Hydrographie, 65(2), 269 - 282. © 2022 IJRAR May 2022, Volume 9, Issue 2 www.ijrar.org ( E - ISSN 2348 - 1269, P - ISSN 2349 - 5138 ) IJRAR22B2543 International Journal of Research and Analytical Reviews (IJRAR) www.ijrar.org 772 [17] Patil, V. P. (2013). Limnology and biodiversity studies of algal flora of Sulwade barrage of Dhule district. [18] Patric, R., & Reimer, C. W. (1996). The Diatoms of the United States. vol. 1. The Aca demy of Natural Sciences Philadelphia Pennsylvania, 688. [19] Philipose, M. T. (1967). Chlorococcales. – 365 pp. ICAR, New Delhi. [20] Prescott, G. W. (1962). Algae of the western great lakes area. WM. C. [21] Prescott, G. W., & Vinyard, W. C. (1965). Ecology of Alaskan freshwater algae V. Limnology and flora of Malikpuk Lake. Transactions of the American Microscopical Society, 84(4), 427 - 478. [22] Randhawa, M. S. (1959). Zygnemataceae. ICAR, New Delhi. Biotechnology Research Communication, 2, 5 - 32. [23] Sarode, P. T., & Kamat, N. D. (1984). Freshwater diatoms of Maharashtra. [24] Shantilal, J. D., Hari, A. N., & Nanabhau, P. (2017). Limnological profile of Sonvad project dam of Dhule district of Maharashtra, India. IJAR, 3(1), 174 - 176. [25] Singh, V. P. (1960, January). Phytoplankton ecology of the inland waters of Uttar Pradesh. In Proc. Symp. Algal (pp. 243 - 271). [26] Whitton, B. A. (1969). Seasonal changes in the phytoplankton of St. James's Park Lake, London. London Natur. [27] Zafar, A. R. (1964). On the ecology of algae in certain fish p onds of Hyderabad - India. Hydrobiologia, 23(1), 179 - 195.