RAPID for platelet enrichment What is RAPID? We thank IIT Bombay for travel support, IMPRINT for project funds, Tata Centre for Technology and Design’s workshop for prototype machining, nanofabrication facility for lithography, and confocal facility (IRCC) for imaging. Towards a portable radial pillar device (RAPID) for clog-free continuous cell separation RAPID for CTC enrichment Where are we currently? • We have demonstrated clogging-free operation of RAPID for ~ 6 hours using whole blood. • A portable prototype has been developed to eliminate the need for syringe pumps. • Clogging-free operation without buffer flow in a passive pillar-based microfluidic chip • Can separate cells from whole blood without any pre-processing step • Operated the device continuously with whole blood for up to 6 hours Microscope image Operating principle Centrifugal prototype Operating principle Performance metrics Inlet Platelet outlet after separation Microscope image Contact: debjani.paul@iitb.ac.in • ~ 60X enrichment of platelets at low flow rates • ~ 95% recovery of platelets at all flow rates • Validated centrifugal prototype with beads • Developing the system to handle higher sample volumes Motor Coupler Chip holder Base Ninad Mehendale, Shilpi Pandey, Oshin Sharma, Misal Choudhary and Debjani Paul Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, INDIA Performance metrics